PRODUCT CLASSIFICATION
·九游·产品分类TECHNICAL ARTICLES
相关文章·九游·产品展示/ Product display
●Purchase●DescriptionThePCSK9(His)-LDLRBindingAssayKitisanELISA-type96-wellformatassaydesignedforscreeningandprofilingpurposes
联系电话:13829799985
The PCSK9(His)-LDLR Binding Assay Kit is an ELISA-type 96-well format assay designed for screening and profiling purposes. Moreover, two pre-formulated assay buffers are supplied to validate PCSK9-LDLR binding affinity in either neutral or acidic binding conditions. The assay takes advantage of the high sensitivity of detection of His-tag PCSK9 by Anti-His HRP-antibody. First, the LDLR ectodomain is coated on a 96-well plate. Next, PCSK9 is incubated with LDLR on the plate. Finally, the plate is treated with Anti-His HRP-antibody followed by addition of an HRP substrate to produce chemiluminescence, measured using a chemiluminescence reader.
Figure 1: Illustration of the ELISA-based PCSK9(His)-LDLR Binding Assay Kit.